Growing Dictyostelium

I have a question to people who has isolated Dicties from the nature. I cannot separate the strains from the fungi. Could anybody give me some advices? Thank a lot
-Joanna Laszczuk, University of Gdansk, POLAND, 30 Nov 1999

• You might try growing the amoebae on a bacterial lawn on a plate containing about 10-40 µ/ml of the polyene antibiotic nystatin. Fungi and yeasts in general are more sensitive to nystatin than cellular slime mould amoebae. The nystatin stock solution (say 20 mg/ml) should be made in dimethyl formamide. Good luck
  -D P Kasbekar, 30 Nov 1999

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