Page Contents: Abstract | Summary Chart

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Muller, I., Subert, N., Otto, H., Herbst, R., Ruhling, H., Maniak, M., Leippe, M., (2005) ' A Dictyostelium mutant with reduced lysozyme levels compensates by increased phagocytic activity. ' J Biol Chem 280 10435-43

 

Abstract:Lysozymes are bacteria-degrading enzymes and play a major role in the immune defense of animals. In free-living protozoa, lysozyme-like proteins are involved in the digestion of phagocytosed bacteria. Here, we purified a protein with lysozyme activity from Dictyostelium amoebae, which constitutes the founding member, a novel class of lysozymes. By tagging the protein with green fluorescent protein or the Myc epitope, a new type of lysozyme-containing vesicle was identified that was devoid of other known lysosomal enzymes. The most highly expressed isoform, encoded by the alyA gene, was knocked out by homologous recombination. The mutant cells had greatly reduced enzymatic activity and grew inefficiently when bacteria were the sole food source. Over time the mutant gained the ability to internalize bacteria more efficiently, so that the defect in digestion was compensated by increased uptake of food particles.
Status:	Published	Type:	Journal article	Source:	PUBMED	PubMed ID:	15640146

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	Genes addressed in this paper
	alyA	alyB	alyC	alyD-1	alyD-2	lyC2	lyC3	lyC4
Topics in this paper
Protein Physical Properties	X	X	X	X	X
Protein Functional Domain	X
Strains/Constructs	X
Non-mammalian Gene Related	X	X	X	X	X	X	X	X
Cellular Location	X
Function/Process	X
Endocytosis	X
DNA/RNA Sequence Features	X
Mutants/Phenotypes	X
Protein/Nucleic Acid Structure						X	X	X

	Genes addressed in this paper
	lyEh1	lyEh2	lyT1-4	lyT2-4
Topics in this paper
Protein Physical Properties
Protein Functional Domain
Strains/Constructs
Non-mammalian Gene Related	X	X	X	X
Cellular Location
Function/Process
Endocytosis
DNA/RNA Sequence Features
Mutants/Phenotypes
Protein/Nucleic Acid Structure	X	X	X	X